Thursday, September 07, 2006
Back to lab
After couple of weeks of sitting at my desk and trying to write my thesis, I decided to run more sequencing gels. Why? - you ask.
Our lab is currently preparing a manuscript on regulation of sxy gene in H. influenzae and we would like to present the data showing that sxy RNA secondary structure limits the sxy expression.
One way of indirectly showing this is to do the mapping of the secondary structures of RNAs in the wt sxy and of RNAs of sxy hypercompetent and non (or hypo) competent mutants. Both of these mutants carry point mutations that affect the stability of their RNA secondary structures.
The analyses that I did so far confirm that the secondary structure of sxy RNA is less stabile in the hypercompetent mutant and more stabile the hypocompetent mutant. Eventhough I got nice and reproducible results (with nuclease mapping of secondary structures), I still do not have the one -? perfect and publishable picture.
This week I will try to produce a nice gel picture showing the differences in the sxy RNA secondary structure in these different RNA species. Although this sounds as a really simple thing to do, I can easily assure you is highly stressful procedure!
Yesterday I prepared the RNA samples and today I am running them in a gel. Hopefully we'll get some very nice results tomorrow!!
Our lab is currently preparing a manuscript on regulation of sxy gene in H. influenzae and we would like to present the data showing that sxy RNA secondary structure limits the sxy expression.
One way of indirectly showing this is to do the mapping of the secondary structures of RNAs in the wt sxy and of RNAs of sxy hypercompetent and non (or hypo) competent mutants. Both of these mutants carry point mutations that affect the stability of their RNA secondary structures.
The analyses that I did so far confirm that the secondary structure of sxy RNA is less stabile in the hypercompetent mutant and more stabile the hypocompetent mutant. Eventhough I got nice and reproducible results (with nuclease mapping of secondary structures), I still do not have the one -? perfect and publishable picture.
This week I will try to produce a nice gel picture showing the differences in the sxy RNA secondary structure in these different RNA species. Although this sounds as a really simple thing to do, I can easily assure you is highly stressful procedure!
Yesterday I prepared the RNA samples and today I am running them in a gel. Hopefully we'll get some very nice results tomorrow!!
# posted by Okapia Johnstoni @ 10:47 AM 
